Whilst not in-depth by any means, this post should hopefully help you understand how Load Balancing works on NetScaler and how some of the different options affect where your requests end up at the back-end.
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load balancing
Azure Multi-Factor Authentication with NetScaler Unified Gateway
MFA/Azure Multi Factor Authentication (previously PhoneFactor) is a multi-factor authentication technology that can be used with IIS, VPNs, OWA, ADFS, Office 365 and NetScaler to name a few using either the LDAP or RADIUS protocols from Azure cloud or on-premise. MFA has the ability to verify a users identity by calling their phone, texting their phone or using an app for verification. Using a phone for the second factor allows you to make use of something everyone will likely already have without additional bits of hardware whilst making it a great deal harder for attackers to break into accounts.